By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis 通過采用gfp標記技術,我們觀察到gfp - cam與胞質分裂期細胞內形成的特征結構存在共分布。
But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in " zone - shaped area " . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique 但是目前對新發現的囊泡膜mu轉運體一dnn樣陽性終末與帶狀區內pag樣陽性神經元之間ej關系,以及谷氨酸脫發酶( gad ,是gaba能神經元和終末的特異性標識物)是否參與其調控作用,尚缺乏系統的形態學資料。
The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation . 2 . following lps or seb was administered intraperitoneally , the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice . double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin 二、小鼠腹腔內給予細菌內毒素lps或腸毒素seb ,用免疫組織化學方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達,并采用雙標記技術觀察了1型il刁受體陽性神經元和加壓素及催產素表達的關系。
The amplification system was optimized so that the pcr with different primers can be carried out under the same condition . the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques . sequences were analyzed and compared base on sequencing analysis3 . 4 and seq / ede software 方法根據mtdna控制區及其周圍區域的序列,設計多對引物,探索優化擴增體系,使擴增條件能夠同時滿足多對引物的需要,用sanger末端終止法及熒光標記技術對樣本進行dna測序, sequencinganalysis3 . 4和seq ede軟件進行序列分析和比對。
The characteristics of this method are : a , directly counting cell number without the influence of the metabolic state of the cells ; b , discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c , less treatment step , and free - radioactivity ; d , high sensitivity and reliability . 2 , using the above assay , immunofluorescent labeled technique , and flow cytometry , the pbmc proliferation , apoptosis , necrosis , cell cycle , activation , cytokines and membrane marker were detected . the results showed that the number of pbmc reduced , but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle , but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased 結合以上創建的方法和免疫熒光流式細胞術,用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養以模擬體內微環境,然后分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究,結果發現用腫瘤上清培養的pbmc細胞數量下降明顯,但同時對其有激活作用,且呈劑量依賴性;細胞數的下降主要是由細胞壞死和凋亡引起的,腫瘤上清對細胞周期沒有阻斷作用,反而略有促進作用; t細胞亞群比例增加,并促進表達th1 、 th2細胞因子。
The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique . the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium , but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure . according the above result , the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end 這種親水表面不僅有利于細胞懸液和培養介質的進入,并可以通過peo橋聯的氨基酸( rgd )為細胞在三維多孔支架內的生長提供類細胞外基質環境;根據以上結果,本文對堿性氨基酸為peo鏈端基的兩親共聚物-氨基酸類細胞外基質修飾的聚乳酸三維支架進行了細胞相容性的測試。
However , the advance of intracellular labeling techniques enables us not only to visualize more complete dendritic arbor for qualitative analysis , but also to examine the relation between changes in the dendritic arborization and the evoked fast postsynaptic curents - 3 - ( fpscs ) in the same neurons during the postnatal development the aim of this study was to systematically examine the postnatal changes in the configuration of fpscs evoked by the focal stimulation of the stratum radiatum of the ca1 region , and the relationship between the dendritic arborization and evoked fpscs in the rat hippocampal ca1 pyramidal neurons using whole - cell blind patch recording technique combined with biocytin intracellular labeling during the postnatal development ( postnatal day 2 - 70 , p2 - p70 ) 但是,細胞內染色技術的進步使我們不僅能觀察到更完整的樹突分支來用于定性研究,而且也可以在同一神經元上研究在發育過程中樹突分支的變化與誘發的快突觸后電流( fastpostsynapticcurrents , fpscs )之間的關系。因此,本研究應用盲法腦片膜片鉗記錄并結合biocytin細胞內染色方法,對發育過程中(生后2 70天)局部刺激大鼠海馬ca1區輻射層在錐體神經元誘發的fpscs的成分變化,以及ca1錐體神經元的樹突分支與誘發的fpscs的關系進行了較為系統的研究。